| 质粒名称: | pC003 - LshC2C2 locus into pACYC184 for spacer cloning |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 79152 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pACYC184 |
| 载体出品公司: | -- |
| 载体抗性: | 氯霉素 |
| 空载体大小: | 4723 bp |
| 完整质粒大小: | 10444 bp |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | LshC2c2 locus |
| 物种来源: | Leptotrichia shahii |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pC003 - LshC2C2 locus into pACYC184 for spacer cloning was a gift from Feng Zhang (Addgene plasmid # 79152 ; http://n2t.net/addgene:79152 ; RRID:Addgene_79152) |
| 参考文献部分: | C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, Zhang F. Science. 2016 Jun 2. pii: aaf5573. 10.1126/science.aaf5573 PubMed 27256883 |
pC003 - LshC2C2 locus into pACYC184 for spacer cloning
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pC003 - LshC2C2 locus into pACYC184 for spacer cloning 10392 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION LshC2c2 locus in pACYC184 with BsaI sites for spacer cloning.
ACCESSION .
VERSION .
KEYWORDS pC003 - LshC2C2 locus into pACYC184 for spacer cloning
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10392)
AUTHORS Abudayyeh OO, Gootenberg JS, Konermann S, Joung J, Slaymaker IM, Cox
DB, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K,
Regev A, Lander ES, Koonin EV, Zhang F
TITLE C2c2 is a single-component programmable RNA-guided RNA-targeting
CRISPR effector.
JOURNAL Science. 2016 Jun 2. pii: aaf5573.
PUBMED 27256883
REFERENCE 2 (bases 1 to 10392)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..10392
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(1475..2665)
/codon_start=1
/gene="tet"
/product="tetracycline efflux protein"
/label=TcR
/note="confers resistance to tetracycline"
/translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
primer_bind 2294..2313
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
CDS 3062..7231
/codon_start=1
/product="type VI CRISPR effector from Leptotrichia shahii
with RNA-guided RNAse activity (Abudayyeh et al., 2016)"
/label=LshC2c2
/translation="MGNLFGHKRWYEVRDKKDFKIKRKVKVKRNYDGNKYILNINENNN
KEKIDNNKFIRKYINYKKNDNILKEFTRKFHAGNILFKLKGKEGIIRIENNDDFLETEE
VVLYIEAYGKSEKLKALGITKKKIIDEAIRQGITKDDKKIEIKRQENEEEIEIDIRDEY
TNKTLNDCSIILRIIENDELETKKSIYEIFKNINMSLYKIIEKIIENETEKVFENRYYE
EHLREKLLKDDKIDVILTNFMEIREKIKSNLEILGFVKFYLNVGGDKKKSKNKKMLVEK
ILNINVDLTVEDIADFVIKELEFWNITKRIEKVKKVNNEFLEKRRNRTYIKSYVLLDKH
EKFKIERENKKDKIVKFFVENIKNNSIKEKIEKILAEFKIDELIKKLEKELKKGNCDTE
IFGIFKKHYKVNFDSKKFSKKSDEEKELYKIIYRYLKGRIEKILVNEQKVRLKKMEKIE
IEKILNESILSEKILKRVKQYTLEHIMYLGKLRHNDIDMTTVNTDDFSRLHAKEELDLE
LITFFASTNMELNKIFSRENINNDENIDFFGGDREKNYVLDKKILNSKIKIIRDLDFID
NKNNITNNFIRKFTKIGTNERNRILHAISKERDLQGTQDDYNKVINIIQNLKISDEEVS
KALNLDVVFKDKKNIITKINDIKISEENNNDIKYLPSFSKVLPEILNLYRNNPKNEPFD
TIETEKIVLNALIYVNKELYKKLILEDDLEENESKNIFLQELKKTLGNIDEIDENIIEN
YYKNAQISASKGNNKAIKKYQKKVIECYIGYLRKNYEELFDFSDFKMNIQEIKKQIKDI
NDNKTYERITVKTSDKTIVINDDFEYIISIFALLNSNAVINKIRNRFFATSVWLNTSEY
QNIIDILDEIMQLNTLRNECITENWNLNLEEFIQKMKEIEKDFDDFKIQTKKEIFNNYY
EDIKNNILTEFKDDINGCDVLEKKLEKIVIFDDETKFEIDKKSNILQDEQRKLSNINKK
DLKKKVDQYIKDKDQEIKSKILCRIIFNSDFLKKYKKEIDNLIEDMESENENKFQEIYY
PKERKNELYIYKKNLFLNIGNPNFDKIYGLISNDIKMADAKFLFNIDGKNIRKNKISEI
DAILKNLNDKLNGYSKEYKEKYIKKLKENDDFFAKNIQNKNYKSFEKDYNRVSEYKKIR
DLVEFNYLNKIESYLIDINWKLAIQMARFERDMHYIVNGLRELGIIKLSGYNTGISRAY
PKRNGSDGFYTTTAYYKFFDEESYKKFEKICYGFGIDLSENSEINKPENESIRNYISHF
YIVRNPFADYSIAEQIDRVSNLLSYSTRYNNSTYASVFEVFKKDVNLDYDELKKKFKLI
GNNDILERLMKPKKVSVLELESYNSDYIKNLIIELLTKIENTNDTL"
rep_origin 9000..9545
/direction=RIGHT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind 9446..9465
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 9662..9679
/label=L4440
/note="L4440 vector, forward primer"
promoter 10071..10173
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
CDS 10174..441
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(10240..10259)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
1 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
61 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
121 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
181 ttccctaaag ggtttattga gaatatgttt ttcgtctcag ccaatccctg ggtgagtttc
241 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
301 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
361 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
421 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
481 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg
541 acccggtcgt cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta
601 ccggtttatt gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt
661 ttgctcaggc tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc
721 agagcctgat aaaaacggtt agcgcttcgt taatacagat gtaggtgttc cacagggtag
781 ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctt gtttcggcgt
841 gggtatggtg gcaggccccg tggccggggg actgttgggc gctgccggca cctgtcctac
901 gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca
961 ccggaaggag ctaccggcag cggtgcggac tgttgtaact cagaataaga aatgaggccg
1021 ctcatggcgt tgactctcag tcatagtatc gtggtatcac cggttggttc cactctctgt
1081 tgcgggcaac ttcagcagca cgtaggggac ttccgcgttt ccagacttta cgaaacacgg
1141 aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt
1201 cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct
1261 agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg
1321 cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa
1381 gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg
1441 gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg
1501 caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca
1561 acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccaatga
1621 tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat
1681 ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa
1741 gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca
1801 gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg
1861 gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc
1921 cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg
1981 gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca
2041 tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac
2101 gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga
2161 gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca
2221 cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc
2281 gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg
2341 tgatgccggc cacgatgcgt ccggcgtaga ggatccacag gacgggtgtg gtcgccatga
2401 tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc
2461 ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta
2521 gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg
2581 gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga
2641 cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg
2701 ataaactacc gcattaaagc tcgtttaaca gctgggaaaa tgtggaattt gaaacaaatt
2761 atcaggatga aagatttatt gatttggtaa cagaaggcag aacatttaga acaggttcaa
2821 atgggaaaat taagttggat ctaaaggcga aagaaggatt gattttacga aaagtaagga
2881 tagatgggga ttatgagtaa atagaagtag ggagctatgt ttgacggctc cttatttttt
2941 tgagatttaa tattgatgtg gtatactaaa aatggaaaat tgtatttttg attagaaaga
3001 tgtaaaattg atttaattta aaaatatttt attagattaa agtagaaacg aggagattgg
3061 aatgggaaat ttatttggac ataagagatg gtatgaagtt cgtgataaaa aagattttaa
3121 aataaaacgg aaagtaaaag ttaaaaggaa ctatgatgga aacaaataca ttttaaatat
3181 taatgaaaat aataataaag aaaaaattga taataataaa tttattagaa aatatattaa
3241 ttacaaaaaa aatgataata ttcttaaaga atttacaaga aaatttcatg caggaaatat
3301 tctatttaag ttgaaaggta aagaaggaat cataagaata gaaaataatg atgatttttt
3361 ggaaacagaa gaagttgtat tatatataga agcatatgga aaatctgaga aattgaaagc
3421 attggggatt acaaaaaaga aaataataga tgaagcaatt aggcaaggaa taactaaaga
3481 tgataaaaaa atagaaataa agagacaaga gaatgaagaa gaaatagaga tagatatcag
3541 agatgagtat actaataaaa cactaaatga ttgttcaata atattaagaa taatagaaaa
3601 tgatgaatta gaaacaaaaa aatcaattta cgaaattttt aaaaatatta acatgagttt
3661 atataagatt atagaaaaaa taattgaaaa tgaaacagaa aaagtatttg aaaatcgata
3721 ttatgaagaa catttaagag aaaaattatt gaaagatgat aaaatagatg ttattttgac
3781 taattttatg gaaataagag aaaaaataaa aagtaattta gaaattctgg gttttgtaaa
3841 attttatctt aatgttggtg gtgataaaaa gaaatctaaa aataaaaaaa tgcttgttga
3901 aaaaatttta aatataaatg tagatttaac agtagaagat atagctgatt ttgttataaa
3961 agagttagaa ttttggaata ttactaaaag aattgaaaaa gtaaaaaaag ttaataatga
4021 atttcttgaa aagagaagaa atagaacata tataaaatct tatgtactat tagataagca
4081 cgaaaaattt aaaatagaaa gagaaaataa aaaagataaa attgtaaaat tttttgtaga
4141 aaatataaaa aataatagta taaaagaaaa aatagaaaaa attttagcag aatttaaaat
4201 agatgaatta ataaaaaaat tagaaaaaga actaaaaaaa ggaaattgtg atacagaaat
4261 ttttggaata tttaaaaaac attataaagt taattttgat tcgaaaaaat tttcaaagaa
4321 atctgatgaa gaaaaagaac tatataaaat catatatcga tatttgaaag gaagaattga
4381 aaaaatattg gtaaatgaac aaaaagtcag attaaagaaa atggaaaaaa tagaaataga
4441 aaaaatttta aatgaaagta tattatctga aaaaatttta aaaagagtta agcaatatac
4501 gttagagcat attatgtatt tagggaaatt aagacataat gatatagata tgacaacagt
4561 aaatactgat gatttttcta gacttcacgc taaagaggaa ttggatttag aattgattac
4621 tttttttgct tctaccaata tggaattaaa taagattttt tctagagaaa atataaataa
4681 tgatgaaaat atagatttct ttggaggaga tcgagaaaaa aattacgtac ttgataaaaa
4741 aattttaaat tcaaaaataa aaataataag agacttagat tttatagata ataaaaataa
4801 cattacaaat aattttatta ggaaatttac aaagatagga acaaatgaaa gaaacaggat
4861 attacatgcg attagcaagg aaagagattt acaaggaacg caagatgact ataataaagt
4921 cataaatata attcaaaatt taaaaatatc agatgaagaa gtgtcaaaag cattaaattt
4981 agatgtagta tttaaagata aaaaaaatat aattactaaa ataaatgata ttaaaatttc
5041 agaagaaaat aataatgata ttaaatattt accatccttt tctaaagtac ttcctgaaat
5101 attaaattta tataggaata atcctaagaa tgaaccattt gatactatag aaacagaaaa
5161 aatagtatta aatgctttaa tatatgtgaa taaagaattg tataagaaat taattttaga
5221 agatgattta gaagaaaatg aaagtaagaa tatattttta caagaattaa agaaaacttt
5281 aggaaacatt gatgaaatag atgaaaatat tatagaaaat tattataaaa atgctcagat
5341 ttctgcttca aaaggtaata ataaagctat taaaaaatat cagaaaaaag taatagaatg
5401 ttatattgga tatttgagaa aaaattatga ggaacttttt gatttttcag attttaaaat
5461 gaatattcaa gaaattaaga aacaaataaa agacattaat gataataaaa cttacgaaag
5521 aataactgta aagactagtg ataaaactat agtaataaat gatgattttg aatatataat
5581 ttcaatattt gcactgttaa atagtaatgc ggttataaat aaaattagaa atagattttt
5641 tgcaacatca gtttggttaa atacttcaga atatcaaaat attatagata ttttagatga
5701 aataatgcaa ttaaatactt tgagaaatga atgtataact gaaaattgga atcttaattt
5761 agaagaattt attcaaaaaa tgaaagagat agaaaaagac tttgatgatt ttaaaataca
5821 aactaaaaaa gaaattttta ataattatta tgaggatata aaaaataata tattaactga
5881 atttaaagat gatattaatg gttgtgatgt tttagagaaa aaattagaaa aaattgtaat
5941 ttttgatgat gaaactaaat ttgagataga taaaaaatcg aatatattac aagatgaaca
6001 aagaaagtta tcaaacataa ataaaaagga tttgaaaaaa aaggttgatc agtatataaa
6061 agataaagat caggaaataa agagtaaaat attgtgtaga ataatattta attctgattt
6121 tttaaaaaaa tacaaaaaag aaatagataa tttaatagaa gatatggaat cagagaatga
6181 aaataaattt caagaaatat attatcctaa agaacgtaag aatgaattat atatttataa
6241 aaagaattta tttttaaata ttggaaatcc aaattttgat aaaatttatg gattaatttc
6301 aaatgatatt aaaatggctg atgcaaaatt tttatttaat attgatggta aaaatattag
6361 aaaaaataaa atatctgaaa ttgatgcaat attgaaaaat ctaaatgata aactaaatgg
6421 atattctaaa gaatacaaag agaagtatat aaaaaaattg aaagaaaatg atgacttttt
6481 tgcaaaaaat atacaaaata aaaactataa atcatttgaa aaagattata atagagtttc
6541 tgaatacaaa aaaattagag atttggtaga atttaattat ttgaataaaa tagaaagtta
6601 tttgatagat ataaattgga aacttgctat tcaaatggct agatttgaaa gagatatgca
6661 ctatattgta aatgggctaa gagaattagg aattattaag ttaagtggct acaatactgg
6721 aataagtaga gcttatccta agcgtaatgg atctgatggc ttttatacaa caacagcata
6781 ctataaattt tttgatgaag aatcttataa aaaatttgag aaaatatgtt atggatttgg
6841 aattgatttg tctgaaaatt ctgaaattaa taagccggaa aatgaaagta ttagaaacta
6901 tatttcacat ttctatattg taagaaatcc gtttgctgat tacagtattg cagaacaaat
6961 tgatagagtt tctaatttat tatcatatag tacacgttat aataattcaa cttatgcaag
7021 tgtatttgaa gtatttaaaa aagatgtgaa tttagattat gatgaattaa agaaaaaatt
7081 taaacttatc ggtaataatg atatacttga aagattaatg aaacctaaaa aagtttctgt
7141 tttagaatta gaaagttata attctgatta tattaaaaac ttgataatag aacttttaac
7201 aaaaatagaa aatacgaatg atacgttata agaatgaaat ttataaggag tatgattaaa
7261 atgcccaata taattcacat aacaagggct gatgatttgt caatatcgaa taatcagctt
7321 ataatggttg atgaagacaa taatgatgag aaaaataaga tttctttgaa tgatatatct
7381 gcgatagtta ttgaaaactg ctattgtaag attagtgcga ttttgcagtt gaggctggtg
7441 gaaaataaca ttccgattat tatttgcaac gagaaacatc agcctgaaat tcattcgctg
7501 ggattattta atcattttca agtgacttta cgaataaatg agcagattga gtgggaaaaa
7561 gagaaaaagg aaaaattgtg gagcaggata gtggagaata agattgaaaa tcaaagagca
7621 ttgttagagt atcttgaaaa aagtgatatt tccattgaaa gactgaaaac atataaggaa
7681 aacttgaaaa aagatgatgc gagtgcagaa catcaggaag caatagcttc aaggatatat
7741 tttcaggaat tgtatagtaa tagttttaaa agatttgatg aagatggagt gaattctgca
7801 cttaattatg gatatatgat tttaagagct ataatatcat caaagattgt agcaaaagga
7861 tttcatccaa gtttaggatt acatcataag tcacagttta atgcctataa tttttctgat
7921 gatataatag aggtttttcg tcctatggtg gattatcttg tttatatgta caaggatatt
7981 ttgaatgaag tgaaattaag taaggaaata aggcaaaaaa tacttcttgt agcacagcag
8041 aaggtattat ttaacaataa gaaatatgat tttttccaag cggtagacta ttatttggat
8101 agtatacgaa attattttgt aaaagatgaa gaagtgataa ttccgacatt gagcgtgatg
8161 gactatgagt attgagtatt ttggtctgct tatgtatgat tttcctatgc agacagatgt
8221 tgagagattt gaatatagga cttttaggaa aaaattgata gaaaagggat attatcagct
8281 tcaaaaatcg gtatatataa tgaggtcgaa aacaaaagaa aaaatcgaac tggcagaaaa
8341 acagttatca atgctcgctc cagaaaattc atcaatccgt tccattatcc tgacagaaga
8401 tcagtttcaa aaatgaaggt acttgcagga gaagtgacaa tgggagaaaa aatttcacaa
8461 aacaaaatat tggaattttg aaaaatcaaa aaatatggta taatagtata gggtggttct
8521 atattattaa taaagagaaa aataggattg aaagtattga aaataaagac aaaatagaaa
8581 aattagaaat aaatatctca tcagcaagaa attaggatgg gatatagacc accccaatat
8641 cgaaggggac taaaacggag accgagattg gtctcggata tagaccaccc caatatcgaa
8701 ggggactaaa actctaaaga attatctatt ctgtctttta aattgatata gaccacccca
8761 atatcgaaaa gtgatattta ataaaaataa cttctgagtg agaataaaat ttcaattctt
8821 gctcattttt tattgttttt tgaatatggt tgcttggttg ttctgaaacg aaaatttttg
8881 gagatgtttt aaatttttag gttgaaaaaa aataagaatt atactataat aaataattat
8941 gcgattaggc taactagagc ctaatttaaa tattttatct gattaataag atgatcttct
9001 tgagatcgtt ttggtctgcg cgtaatctct tgctctgaaa acgaaaaaac cgccttgcag
9061 ggcggttttt cgaaggttct ctgagctacc aactctttga accgaggtaa ctggcttgga
9121 ggagcgcagt caccaaaact tgtcctttca gtttagcctt aaccggcgca tgacttcaag
9181 actaactcct ctaaatcaat taccagtggc tgctgccagt ggtgcttttg catgtctttc
9241 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcggact gaacgggggg
9301 ttcgtgcata cagtccagct tggagcgaac tgcctacccg gaactgagtg tcaggcgtgg
9361 aatgagacaa acgcggccat aacagcggaa tgacaccggt aaaccgaaag gcaggaacag
9421 gagagcgcac gagggagccg ccagggggaa acgcctggta tctttatagt cctgtcgggt
9481 ttcgccacca ctgatttgag cgtcagattt cgtgatgctt gtcagggggg cggagcctat
9541 ggaaaaacgg ctttgccgcg gccctctcac ttccctgtta agtatcttcc tggcatcttc
9601 caggaaatct ccgccccgtt cgtaagccat ttccgctcgc cgcagtcgaa cgaccgagcg
9661 tagcgagtca gtgagcgagg aagcggaata tatcctgtat cacatattct gctgacgcac
9721 cggtgcagcc ttttttctcc tgccacatga agcacttcac tgacaccctc atcagtgcca
9781 acatagtaag ccagtataca ctccgctagc gctgatgtcc ggcggtgctt ttgccgttac
9841 gcaccacccc gtcagtagct gaacaggagg gacagctgat agaaacagaa gccactggag
9901 cacctcaaaa acaccatcat acactaaatc agtaagttgg cagcatcacc cgacgcactt
9961 tgcgccgaat aaatacctgt gacggaagat cacttcgcag aataaataaa tcctggtgtc
10021 cctgttgata ccgggaagcc ctgggccaac ttttggcgaa aatgagacgt tgatcggcac
10081 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
10141 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
10201 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
10261 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
10321 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
10381 catccggaat tc
//
